Asthmatic children that are uncontrolled despite inhaled corticosteroids have a distinct breathprint (the pacman2 study)

Method VOCs were measured in thirty-three asthmatic children (age: 11.8 yrs, SD 2.3 yrs) who participated in the PACMAN2 study. All children were current users of ICS. Current asthma control was assessed using the Asthma Control Questionnaire. Long-term asthma control was based on reported symptoms in the four seasons preceding baseline and follow-up visit. A single vital capacity volume of exhaled air was collected upon 5 minutes of normal breathing through a three-way non-re-breathing valve with a VOC filter in a Tedlar bag. The VOCs in the breath sample were subsequently captured on Tenax GR Tubes and analyzed offline on a validated panel of four eNoses with different sensor technologies (Owlstone Lonestar, Sensigent Cyranose 320, Comon Invent eNose and Tor Vergata TEN). Breathprints were analysed per eNose using principal component analyses (PCA). ROC curves and cross-validated accuracy values of significant (t-test) principal components were used to assess the accuracy of the devices to discriminate between controlled and uncontrolled patients. Additionally, the fraction of exhaled nitric oxide (FeNO) was measured with a NIOX Mino and an expiration time of 6 seconds.